Figure 6
Analysis of comparative cartilage-formation by hES cell-derived paraxial mesoderm-like cells and adult bone marrow-derived hMSCs.
The H9 hES cell-derived KDR−PDGFRα+ paraxial mesoderm (A–C) and human bone marrow-derived STRO1+ MSCs from donor 1 (p4, STRO1+MSC1) (D–G) were subjected to 3D pellet chondrogenesis culture under PT (A, F), PTB (B, G), PT/B (C), T (D) and TB (E) conditions. Four contiguous sections were stained with Toluidine Blue (T-Blue) (i, ii), or immunostained for COL1 (iii), COL2 (iv), or isotype control (v). (ii) shows an enlarged view of the square in (i). Scale bars, 200 μm.
