Figure 1
From: Involvement of RDR6 in short-range intercellular RNA silencing in Nicotiana benthamiana
Local induction and cell-to-cell spread of virus-induced RNA silencing.
The first and second young leaves of N. benthamiana GFP16c and GFP16c/RDR6i at the six-leaf stage were mechanically inoculated with an equal amount of TCV-GFPΔCP RNA transcripts. Control plants were mock-inoculated with 10 mM Tris-HCl (pH 8.0) containing 10 mM EDTA. Both the upper and lower epidermises of inoculated leaves were photographed 8 days post-inoculation (dpi) (e–l) or 12 dpi (a–d, m–t) using a Nikon Coolpix995 digital camera under long-wavelength UV illumination through a yellow Kodak No. 58 filter. Gfp RNA-silenced tissue (foci) showed red chlorophyll fluorescence and gfp-expressing tissue showed green fluorescence. Colour-coded arrows in Panels (g), (h), (o) and (p) show the same gfp silencing foci observed from the upper or lower epidermis. A diagram showing the genome of TCV-GFPΔCP is included. A cartoon of a plant shows the positions of the first and second inoculated leaves (I. L.).
