Figure 2
From: Involvement of RDR6 in short-range intercellular RNA silencing in Nicotiana benthamiana
Influence of RDR6 on intercellular RNA silencing.
(a–b) The RDR6 gene affects the intercellular spread of TCV-GFPΔCP-induced gfp silencing. The average numbers of silencing foci per inoculated leaf for four leaves in a typical experiment using recombinant RNA transcripts produced from 2.5 µg of TCV-GFPΔCP DNA template are shown (a). Numbers of foci were counted 8 days post-inoculation (dpi). The average sizes of 12 randomly selected silencing foci from the upper and lower epidermises were analysed (b). (c–l) Dark gfp RNA silencing foci from the upper and lower epidermises of N. benthamiana GFP16c and GFP16c/RDR6i were examined under a fluorescence microscope using a green filter (c–f) or through bright field illumination (g–l). The boxed areas were used to estimate the numbers of epidermal cells in which gfp-silencing occurred. Individual epidermal cells are outlined and marked with asterisks. Scale bars are indicated.
