Figure 1
From: Environmental conditioning in the control of macrophage thrombospondin-1 production
TSP-1 expression by BM-MΦs is stimulated via TLR4.
(A) BM-MΦs were cultured for 24 hrs in media alone or in the presence of various innate ligands. To control for autofluorescence, TSP-1 production was always determined by comparison with comparably activated TSP-1 KO cells. LPS, but not BLP or CpG induced TSP-1. Results are representative of 3 independent experiments. (B) WT and TLR4 KO BM-MΦs were cultured in media alone or stimulated with LPS or ultrapure LPS for 24 hr and assayed for NO and TSP-1 production. Results are the averages of 3 separate experiments. ** p = <0.01, Mann-Whitney two-tailed test.
