Figure 4
From: Environmental conditioning in the control of macrophage thrombospondin-1 production
Peritoneal macrophages produce a soluble mediator that increases TSP-1 expression.
BM-MΦ or Peritoneal MΦs (P-MΦ) were cultured in media alone or stimulated with LPS. (A) P-MΦ produced greater amounts of TSP-1 as indicated by increased TSP-1+ percentage (B) TSP-1 protein levels were higher in P-MΦ. (C) About 28% of WT BM-MΦ, co-cultured with TSP-1 KO BM-MΦ (ratio 1:1) producedTSP-1. This increased to 48% when WT BM-MΦ were co-cultured with TSP-1 KOP-MΦ.
