Figure 5
From: Environmental conditioning in the control of macrophage thrombospondin-1 production
LPS in combination with adenosine or PGE2 increase TSP-1 expression.
(A) BM-MΦs were cultured in media alone or stimulated with adenosine or CGS (a selective A2A agonist) alone or in combination with LPS. Adenosine and CGS alone did not up-regulate TSP-1 expression. The addition of Adenosine to LPS-stimulated cultures increased TSP-1 expression by about 1.5 fold, while the addition of CGS to LPS-stimulated culture increased TSP-1 expression 2 fold. (B) WT and IL-10 KO BM-MΦs were cultured in media alone or stimulated with LPS or LPS & PGE2 for 48 hrs. The addition of PGE2 increased TSP-1 expression 2-fold. (C) WT and TNFR1 KO BM-MΦs were cultured in media alone or stimulated with LPS for 48 hrs. The TSP-1 response is independent of the presence of TNFR1. Results are the averages for two independent experiments. * p = <0.05 Mann-Whitney U test. (D) WT BM-MΦs were stimulated with LPS + CGS for 24 hrs followed by the addition of IFN-γ, IL-4, IL-17, or IFN-γ & IL-17 for a further 24 hrs. IFN-γ and IL-4 reduced TSP-1 expression by 30-50%. IL-17 had no effect. Results are the averages from two independent experiments. *p = <0.05 Mann-Whitney U test.
