Figure 7

TSP-1 expression during acute inflammation segregates to the macrophage population and is reduced in the presence of activated T cells.

Animals with Endotoxin-Induced Uveitis were sacrificed at different time points after disease induction. Cells released from the eyes were analysed by flow cytometry. (A) TSP-1 expression in macrophages (CD45⁺, CD11b⁺, 1A8(Ly6G)^- cells) at 12 hours after treatment. (B) CD45⁺ cell infiltrate was detectable throughout the course of the experiment and peaked at 36 hours. WT and TSP^KO animals had similar levels of infiltrate (C) The level of TSP-1 production fell rapidly and significantly over the first 60 hours of the disease course (D) The T cell content of the infiltrate increased significantly with time to 60 hours after the induction of disease. Results are representative of two individual experiments, n = ≥3 mice. *p = <0.05 Mann-Whitney U test.