Figure 3
From: The Bcl-2 family member BIM has multiple glaucoma-relevant functions in DBA/2J mice
Bim deficiency delays RGC death after CONC.
(A) RGC death, as judged by presence of cleaved caspase 3 (cCASP3+), was clearly decreased in both Bim+/- and Bim-/- mice 3 days after injury (the onset of cell death). (B) Quantification of cCASP3+ cells shows that there is a significant decrease (*, P<0.01) in cell death in both Bim+/- and Bim-/- mice at 3 and 5 days after injury. Interestingly, 14 days after injury, when about 50% of RGCs are lost, cell death is increased in the Bim-/- mice compared to the other genotypes (cCASP3+ cells per mm2: Bim+/+ 6±1; Bim-/- 19±3; P = 0.01). N≥6 for each genotype and time point; scale bar, 100 μm.
