Figure 1 | Scientific Reports

Figure 1

From: Sex determination in beetles: Production of all male progeny by Parental RNAi knockdown of transformer

Figure 1

(A) Schematic representation of isoforms of Tctra pre-mRNA showing the primer positions and region used for the preparation of dsRNA.Boxes show exons and lines show introns and the numbers in the exons show sizes (bp). Common regions are shown in blue and male-specific regions are shown in red color. Green horizontal bar shows the region used to prepare Tctra dsRNA. Black dots represent the presence of stop codons in male-specific exons. Arrows represent the primer locations. Location of putative Tra/Tra2 binding sites are marked using green and yellow vertical lines. RBP1 binding sites are shown using red vertical lines. ISS sites are shown by black triangles. Primers shown as F and female-specific R were used for quantifying female-specific isoform. Primers shown as male-specific F and R2 were used for quantifying male-specific isoforms. (B) Gel picture showing the amplicons generated by PCR using sex-specific c-DNA as templates and F and R1 primers of Tctra.

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