Figure 2 | Scientific Reports

Figure 2

From: Sex determination in beetles: Production of all male progeny by Parental RNAi knockdown of transformer

Figure 2

Sex-specific splicing of Tcdsx (AC&E) and Tctra (BD&F) in Tctra or malE dsRNA injected insects.

Tctra or malE dsRNAs were injected into newly emerged (A&B), 48 hr-old adults (C&D) or newly ecdysed pupa (E&F). RNAs were isolated on 5th day after injections and the mRNA of Tcdsx and Tctra were detected by resolving RT-PCR products on agarose gel. Female and male adults were injected with Tctra or malE dsRNAs soon after adult emergence. The ovaries (G&H) or testes (I&J) from malE (G&I) or Tctra (H&J) dsRNA injected insects were dissected on 7th day after adult emergence, stained with acridine orange and photographed using a fluorescent microscope.

Back to article page