Figure 4
(A) Relative expression of female (Tctraf) and male-specific (Tctram) isoforms of Tctra in unfertilized (0–12 hr after egg laying (unmated females laid fewer eggs therefore, 12 hr collection was necessary to obtain enough eggs) and fertilized eggs (0–5 hr after egg laying).Eggs laid over 0–12 hr period by unmated females and 0–5 hr period by mated females were collected, total RNA was isolated and the mRNA levels of female- and male-specific isoforms of Tctra were quantified by qRT-PCR. Higher levels of female-specific Tctra mRNA were detected in both fertilized and unfertilized eggs. (B &C) Relative change in the expression of female-specific isoform (B) and male-specific isoform (C) of Tctra during the embryonic development. Eggs laid by mated females over an hour period were collected and incubated at 30°C. Samples were collected at 2 hr intervals until 12 hr after egg laying. Similarly, eggs laid by mated females over 5 hr period were collected and incubated at 30°C. Samples were collected at 12 hr intervals until hatching. Total RNA was isolated and the mRNA levels of female- and male-specific isoforms of Tctra were quantified by qRT-PCR. A peak of female-specific isoform of Tctra is detected at 12–13 hr after egg laying and a peak of male-specific isoform of Tctra is detected at 18–23 hr after egg laying.
