Figure 1
From: Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA
iPSC Colonies Derived with M3O-Based mRNA Reprogramming Cocktail.
(a) Phase contrast imagery showing morphology of an iPSC colony picked from the first M3O-based BJ reprogramming plate and replated onto feeders. (b) Immunostaining of an expanded clone revealing expression of cell-surface and nuclear pluripotency markers. (c) Immunofluorescence images showing expression of neuro-ectoderm markers after in vitro differentiation of the iPSC clone shown in the previous panel. (d) Immunostaining showing expression of the endodermal marker Sox17 in cells derived from the same clone subjected to in vitro endodermal differentiation. (e) α-Actinin immunostaining of cardiomyocytes derived by mesodermal differentiation of the same iPSC clone (see also Supplementary Movie 1).
