Figure 2

Comparison of Reprogramming Efficiency Using 4 Different mRNA Cocktails.

(a) Flowchart summarizing the feeder-based 4-cocktail comparison experiment described in the text. The 5-factor reprogramming cocktails comprised wild-type Oct4 or M₃O plus Sox2, Klf4, c-Myc-T58A and Lin28 mRNA transcripts, while 6-factor cocktails included these RFs plus Nanog. (b) Immunofluorescence imaging showing the yield of TRA-1-60⁺ colonies produced by the four different cocktails at day 18 of the experiment. The total number of days of transfection is indicated for each culture. (c) Chart showing the yield of TRA-1-60⁺ colonies at day 18 versus the number of days of transfection using the four cocktails.