Figure 2
The release of Netrin-1 from vesicles induces either attraction or turning.
(a) DIC images of a GC before VB (−60 s and −10 s) and after VB (30, 60, 120 and 240 s) Black crosses indicate Lv. (b) top: GC profiles at −60 s (green) and −10 s (red) before VB; bottom: GC profiles at -10 s (green) and 240 s (red) after VB. GC before VB explored the environment, but after VB they clearly grew towards Lv. In this experiment the laser pulse completely broke the lipid vesicle. Nv~1,750. (c) as in a, but Nv~950. (d) as in b but profiles obtained from the GC shown in c. (e) Time evolution of the distance (Dist) between Lv and the nearest tip of the GC for 5 different experiments. The vertical broken line indicates the time of VB. Following VB the GC started to grow with a delay varying from 20 to 60 s. Scale bar: 5 μm.
