Figure 4
From: Nitric oxide rescues thalidomide mediated teratogenicity
Nitric oxide recovers angiogenesis.
(A) Representative bright field and DAR images of pre- and post-treated aortic rings. (B) The bright field images were used to calculate the number of tubes after various treatments (n = 5). *p>0.01 vs control; # p>0.01 vs Thal. (C) Fluorescence intensity of aortic rings calculated following incubation with DAR-4M-AM. *p>0.01 vs control; # p>0.01 vs Thal. (D) Embryos were treated with 40 μg thalidomide followed by treatment with spNO (10 μM). Total RNA was harvested from HH17-18 embryo and evaluated for angiogenic genes expression profile. cDNA was primed with avian specific VEGFR2 and Ang1 primers. As an internal control, β-actin mRNA was measured in parallel. Expression of VEGFR2 and Ang1 was analyzed for all treatments. Densitometric analysis of blots relative to β-actin expression. *p <0.05 vs control; #p<0.01 vs Thal.
