Figure 5
From: Nitric oxide rescues thalidomide mediated teratogenicity
Nitric oxide inhibits thalidomide induced caspase-3 dependant apoptosis.
(A–C) Endothelial cells or limb cells or chick aorta or limb buds dissected from a 6th day old (HH17-18) embryo were treated with thalidomide (40 μM) for 4 h spNO (10 μM) was added 5 min after thalidomide treatment. Annexin V and propidium iodide was used to check apoptosis. Apoptotic cells per field (n = 100 fields) were counted from the images taken using a fluorescence microscope. *p<0.05 vs control; #p<0.01 vs Thal. (D) Migration of the limb cells was assayed using scrape wound healing assay. Confluent limb cells were treated with caspase-3 inhibitor DEVD (10 nM) or thalidomide or spNO. A wound was created using a sterile pipette tip and difference in area of the wound was measured before and after 4 h. *p = 0.029 vs Thal; *p<0.01.
