Table 1 Semi-quantitative RT-PCR primers
From: Nitric oxide rescues thalidomide mediated teratogenicity
Gene of Interest | Product size | Annealing Temperature | Primer Sequence |
|---|---|---|---|
eNOS S1179D | 203bp | 63.4°C | Sense- 5′ GAGGATCGTTTCGCATGATT 3′ |
|  |  |  | Anti-Sense – 5′ CTCGTCCTGCAGTTCATTC 3′ |
VEGFR2 | 218bp | 65.4°C | Sense- 5′ CACCATGGTCTGTTCCAGTG 3′ |
|  |  |  | Anti-Sense – 5′ CCATGGCTGCAGTCTCTGTA 3′ |
Angiopoetin1 | 546bp | 58°C | Sense- 5′ TGGAACATGTGATGGAAAAC 3′ |
|  |  |  | Anti-Sense –5′CCTTAGAACAGAGGGTAATTAGAG 3′ |
Caspase-3 | 262bp | 55°C | Sense-5′ CAA ACT TTT TCA GAG GGG ATC G 3′ |
|  |  |  | Anti-Sense –5′GCA TAC TGT TTC AGC ATG GCA 3′ |
FOXO1 | 325bp | 59°C | Sense- 5′ GCAGATCTACGAGTGGATGGTC 3′ |
|  |  |  | Anti-Sense –5′AAACTGTGATCCAGGGCTGTC 3′ |
FOXO3a | 438bp | 59°C | Sense-5′ CTTCAAGGATAAGGGCGACAG 3′ |
|  |  |  | Anti-Sense –5′TCGTCCTGGACTTCATCCAAC 3′ |
β-Actin | 162bp | 60.5°C | Sense- 5′ TCTGACTGACCGCGTTACTC 3′ |
|  |  |  | Anti-Sense – 5′ CCATCACACCCTGATGTCTG 3′ |
GAPDH | 510bp | 58°C | Sense- 5′ TCAACGGATTTGGTCGTATT 3′ |
|  |  |  | Anti-Sense –5′ CTGTGGTCATGAGTCCTTCC 3′ |
