Figure 8
AAV-mediated transgene delivery to the adult murine hippocampus for bidirectional manipulation of GM-CSFRα levels in wildtypes.
(A) Vectors employed in this study. (B) Representative example of robust hippocampal reporter gene expression (AAV-UNC; CA1 region). Reference bar, 50 µm. (C) GM-CSFRα levels in western blots of AAV-treated hippocampal samples differ in the expected construct-dependent manner. (D) Quantification of blot in (B). (**, p<0.01) CBA, chicken beta actin promoter; GMRα, GM-CSF receptor alpha subunit; IRES, internal ribosome entry site; eGFP, enhanced green fluorescent protein; U6, human U6 promoter; shRNA, small hairpin RNA; hrGFP, humanized renilla green fluorescent protein; UNC, universal negative control. Data is presented as mean ± SEM.
