Figure 4
From: Alzheimer brain-derived tau oligomers propagate pathology from endogenous tau
Brain-derived tau oligomers are potent seeds inducing the aggregation and propagation of endogenous tau in vivo.
Only mice injected with brain-derived tau oligomers displayed significant widespread tau pathology 11 months post injection. Tau pathology was detected using well established methods, including ThS and Gallyas silver, as well as immunohistochemistry using AT8 and HT7 antibodies. (a–f) NFT in the hippocampal CA1 region and frontal cortex. Positive staining with Gallyas silver, ThS and AT8 (specific for Ser202/Thr205 phosphorylated tau) established the presence of hyperphosphorylated NFT deposits and the spreading of the pathology to areas far from the injection site (hippocampus). (g–j) HT7 staining (specific for human tau) was confined to the injection site (g), demonstrating that tau deposits in neighboring areas are comprised of endogenous murine tau and not derived from the original inocula. Scale bars 12 µm.
