Figure 1
Multiplex epitope mapping using cell-surface display of a multi-target fragment library.
A library of 60 antigen genes is pooled, fragmented by sonication and ligated into a bacterial surface display vector. This multi-target fragment (MTF) library contains approximately 6*105 functionally expressing members. The MTF library is incubated with a mixture of antibodies and positive cells are sorted for DNA sequencing. Inserts are sequenced and aligned to reference genes to determine the antibody epitope.
