Table 2 Summary of MTF and target-specific fragment epitope mappings for three pAbs and two mAbs. In most cases the MTF shows the same epitope as target-specific fragment libraries. N-terminal epitopes for EGFR and PSMA were not detected multiplex MTF. In general, epitopes determined with MTF are larger than those determined with dedicated fragment libraries
Antibody | Target Library | Epitope 1 | Epitope 2 | Epitope 3 | Epitope 4 |
|---|---|---|---|---|---|
FOLH mAb | FOLH fragment | 473-491 | Â | Â | Â |
| Â | MTF | 474-489 | Â | Â | Â |
VEGF mAb | VEGF fragment | 45-50 | Â | Â | Â |
| Â | MTF | 42-70 | Â | Â | Â |
FOLH pAb | FOLH fragment | 231-245 | 253-264 | 287-301 | 317-331 |
| Â | MTF | 231-242 | 254-268 | 284-291 | 321-336 |
| Â | MTF (multiplex) | N.D. | 256-265 | 289-298 | 314-328 |
EGFR pAb | EGFR fragment | 344-352 | 381-398 | 425-442 | Â |
| Â | MTF | N.D. | 381-393 | 404-446 | Â |
| Â | MTF (multiplex) | 346-366 | 372-386 | N.D. | Â |
VEGF pAb | VEGF fragment | 3-8 | 11-16 | Â | Â |
| Â | MTF | 1-10 | 13-26 | Â | Â |
| Â | MTF (multiplex) | 3-9 | 13-25 | Â | Â |
