Figure 1 | Scientific Reports

Figure 1

From: 12-Chemokine Gene Signature Identifies Lymph Node-like Structures in Melanoma: Potential for Patient Selection for Immunotherapy?

Figure 1

Interrogation of a 12-chemokine gene expression signature across solid tumors of differing histology: Principal component analysis (PCA).

Gene expression microarrays on 14,492 samples (primaries and metastases) were examined and total PCA score was derived as a measure of chemokine signal. The top 10% of samples were selected as high. For this study, we calculated a PCA score based on PC1 that approximates an average Z-score based on the log2 intensities for each of the 12 chemokine genes in the gene expression signature. RNA was extracted from tumors frozen within 15 minutes of surgical removal (according to strict standard operating procedures). *The 569 skin tumor samples include non-melanomas (i.e. basal cell, squamous cell and Merkel cell carcinomas), primary melanomas, as well as 120 non-locoregional metastases from stage IV patients. **Compared to all other samples, RNA was extracted from paraffin of suboptimal amounts of tumor tissue.

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