Figure 11
From: Use of Direct Gradient Analysis to Uncover Biological Hypotheses in 16S Survey Data and Beyond
Direct gradient analysis of CD45+ interstitial epithelial leukocytes during C. difficile infection.
A. CD45+ IEL from untreated mice and infected mice were constrained by infection status using CCA and plotted using rgl. Blue, cells from untreated mice; red, cells from infected mice. B. Boxplots characterizing relative fluorescent intensities of the cells in the region indicated in panel A by the white ellipse. The vertical axis depicts cell frequency, relative to all CD45+ events; FSC, forward scatter; SSC, side scatter. Figure 11C depicts the frequency of cells in both the uninfected and infected state (as a % of CD45+ cells ) within the selected region.
