Figure 1 | Scientific Reports

Figure 1

From: Loss of the respiratory enzyme citrate synthase directly links the Warburg effect to tumor malignancy

Figure 1

Human cancer cell lines display a wide spectrum of CS expression.

(A) Western blotting of CS expression in various cancer cell lines. Total proteins isolated from cells as indicated were blotted with antibodies for CS and β-actin. (B) Western blotting of CS in several cervical cancer cell lines. Total proteins isolated from cells as indicated were blotted with antibodies for CS and β -actin. ‘Normal’ refers to normal primary cervical epithelial cells; Z172 and Z183A are HPV-immortalized cervical epithelial cells; HeLa, SiHa and Cx are cervical carcinoma cells displaying different malignancy grades. (C) Western blotting of proteins involved in the TCA cycle, glycolysis and bioenergetic homeostasis in HeLa, SiHa, MCF7 and PC-3 cell lines. Total proteins isolated from the four cell clones were blotted with antibodies as indicated. The level of β -actin serves as a loading control.

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