Figure 1
Increased susceptibility and Listeria load in Fpr-deficient mice.
(a) The survival of mice post Listeria infection. Mice were i.v. injected with 2 × 104 Listeria in 100 µl DPBS and observed for up to 10 days. Results shown are the means of three experiments. * significantly reduced survival of Fpr-deficient mice compared with WT littermates, p = 0.031. n = 8 mice for each group in each experiment. (b) Listeria load in the liver. Mouse livers were harvested 3 d after infection and homogenized in DPBS. The tissue suspension was diluted, inoculated and incubated in agar plates at 37°C for 24 h. The bacterial colony forming units (CFUs) were counted. n = 3−4 mice per group in each experiment. * significantly increased Listeria CFUs formed by liver lysates from Fpr-deficient mice compared with WT mice (p = 0.008). Data are the mean ± SD from a representative experiment out of three performed. (c) Neutrophils in the liver of Listeria-infected mice. Mice were i.v. injected with 2 × 104 Listeria in 100 µl DPBS. Neutrophils in the liver were purified and analyzed with flow cytometry at different time points. n = 5 mice per group in each experiment. * significantly decreased neutrophils in the liver of Fpr-deficient mice at all measurement time points as compared with WT mice (p = 0.006). (d) Immunofluorescence staining of infiltrating neutrophils. The livers of mice were cryosectioned and stained with Ly6G (Green) and DAPI (Blue) 6 h and 48 h after Listeria infection (400 ×). (e) Abscess formation in the liver of Listeria-infected mice. Mice were injected with 2 × 104 Listeria and the livers were harvested at 48 h. Paraffin liver sections (5 μm) were stained with H&E. Marked areas delineate the edges of abscesses (200 ×).
