Figure 5

FvC5SD expression in transgenic tomato leads to resistance to infestation by fungus S. sclerotiorum.
(a) The lesion formation in leaves of WT and transgenic lines (D-2, D-11 and D-12) after five days of inoculation. The diseased leaves showed growth of the fungi and lesion formation. Shown in inset is the trypan blue staining of fungal mycelium in one of the infected leaves viewed under light microscope (40× magnification). (b) Time kinetics of disease development in tomato as measured by size of the lesion. Data points represent the mean ±SE of lesion sizes from three leaves. WT, wild type.