Figure 2

Two-photon fluorescence imaging of cortical and hippocampal CA1 pyramidal neurons with 910-nm femto-second and 1030-nm picosecond excitation in a young adult H-line mouse brain.
(a) Maximum intensity projections of three-dimensional stacks were obtained with 910-nm and 1030-nm lasers (b). Four normalized xy frames from the z-stack at various depths are shown, including the dura matter, apical dendrite, soma, white matter and hippocampus, respectively (left panel). The arrowhead shows a part of a basal dendritic process at cortex layer V.