Figure 5

Expression of furin in MDA-MB-468 cells and two-photon laser microscopy images of MDA-MB-468 cells incubated with 2 or 2-Scr.
Western blot analysis (a) and quantification (b) of furin in MDA-MB-468 cells and LoVo cells. Furin was highly expressed in MDA-MB-468 cells (96.2% of GAPDH) while in LoVo cells it was less expressed (24.5% of GAPDH, 4-fold, p = 0.008). (c, d) Immunofluorescence staining of MDA-MB-468 cells with rhodamine-labeled antibody against furin: DsRed channel (red, furin) (c); merged image with DAPI (blue, nucleus) (d). Scale bar: 20 μm. (e, f) TPLM images (λex = 725 nm, λem = 565–636 nm) of MDA-MB-468 cells incubated with 2 (e) or 2-scr (f) at 100 μM for 8 h and then rinsed and fixed prior to imaging. Scale bar: 20 μm.