Figure 7
In vivo imaging MDA-MB-468 tumors with 1.
(a) Representative coronal MR images of mice with subcutaneously xenografted MDA-MB-468 tumors at 0 min, 50 min and 90 min after two intravenous injections of 1 (upper) or 1-Scr (lower) via tail veins (1st injection: 0.15 mmol/kg at 0 min; 2nd injection: 0.15 mmol/kg at 50 min). Tumors are indicated by arrows. (b) Quantitative analysis of grey values of tumor MR images at various time. (c) Western blot analysis and (d) quantification of furin expression in MDA-MB-468 tumors on mice injected with 1, with/without 1-Scr after 240 min of MRI. Furin protein in tumors of mice injected with 1 has a decreased expression level of 54.8% of GAPDH, compared with that in tumors on mice untreated (90.9% of GAPDH, 1.7-fold, p = 0.002). Expression of furin protein in tumors of mice injected with 1-Scr does not show obvious change (106.3% of GAPDH), compared with that in tumors on mice untreated (p = 0.08). (e) Immunofluorescence staining images of tumors on mice untreated (Control), injected with 1 or 1-Scr. Furin is stained red and nucleuses are stained blue with DAPI. Scale bar: 20 μm.
