Figure 6
Measurement of transepithelial resistance (TER) revealed that inhibition of p38 MAPK activation partially preserved the barrier function of tight junction in polarized MDCK cells treated with COM crystals.
The polarized MDCK cells grown in TranswellsTM were pretreated without or with 20 μM SB239063 for 2-h before incubation with 100 μg/ml COM crystals for up to 48-h. TER was serially measured at 0, 3, 6, 9, 12, 24, 36 and 48-h after COM treatment. The polarized MDCK cells grown in COM-free medium served as the controls. N = 3 independent experiments for each data point; * = P<0.005 vs. TER of the controlled cells at the corresponding time-point; ** = P<0.0005 vs. the basal TER level; # = P<0.05 vs. TER of COM-treated cells pretreated with 20 μM SB239068 at the corresponding time-point.
