Figure 8
Semiquantitative fluorescence assay revealed that COM crystals induced intracellular hydrogen peroxide production.
The polarized MDCK cells were pretreated without or with 20 μM SB239063 for 2-h and then incubated with 100 μg/ml COM crystals for 48-h. Subsequently, DHR123 was added into the culture medium and the fluorescence signal was detected by fluorescence microscope (upper panels). The mean florescence intensity was then quantitated (lower graph). N = 3 independent experiments of each bar; * = P<0.001 vs. control; ** = P<0.05 vs. control; # = P<0.05 vs. COM-treated group.
