Figure 1

New chemicals that target an insect-specific cysteine of AP-AChEs.
Upper left panel: cross-section of the AP-AChE and AChE active sites showing the locations of the insect-specific cysteine and the corresponding residue in non-insect species; upper right panel: chemical structures of PMn, PYn, PYS18 and PMS20; middle panel: two-step quiescent affinity labeling mechanism for PMn and PYn and definition of kinetic parameters; lower panel: syntheses of PMn, PYn, PYS18 and PMS20. DCM: CH2Cl2; DMF: N,N′-dimethylformamide; NBS: N-bromosuccinimide; TFA: CF3CO2; THF: tetrahydrofuran; : reflux.