Figure 6
Determination of equilibrium dissociation constants (Ki).
Left: reciprocal hydrolysis rate (1/v in second per optical density) was plotted against reciprocal substrate concentration (1/[ATCh] in 1/mM) in the absence and presence of an inhibitor at varying concentrations; right: the slope of the double reciprocal plot was plotted against inhibitor concentration ([I] in μM). Ki was obtained from the negative x intercept of the slope replot.
