Figure 7
Interactions between H68A/Y69A/G71A/H72A/E73A mutant of SP0498 Big domain and Ca2+ measured by Stains-all and ITC assays.
(A) CD spectrum of this mutated SP0498 Big domain mixed with Stains-all dye at molar ratios of 10:1. (B) Titration of this mutated SP0498 Big domain with Ca2+ was performed in 20 mM HEPES buffer pH 6.5 (containing 100 mM NaCl) measured by ITC at 20°C. The upper thermogram panel shows the observed heats for each injection of CaCl2 at 120 s intervals after baseline correction whereas the lower panel depicts the binding enthalpies vs. Ca2+/protein molar ratio.
