Table 2 Binding constants of SP0498 Big domains and Ca2+ determined by ITC
Mutated Site | Dissociation constants Kd (μM) |
|---|---|
Wild type | 0.24 ± 0.02 |
T4A | 0.32 ± 0.02 |
I8A/E9A | 0.24 ± 0.00 |
S11A/Q12A | 0.26 ± 0.02 |
D17A | 0.19 ± 0.02 |
E28A/G29A/R30A | non-available a |
Y35A/S36A | 0.23 ± 0.01 |
S44A/E48A/G49A/I52A | 0.31 ± 0.04 |
H68A/Y69A/G71A/H72A/E73A | no binding was tested |
H68A/Y69A | 0.19 ± 0.01 |
G71A/H72A/E73A | 0.18 ± 0.01 |
