Figure 3 | Scientific Reports

Figure 3

From: Feedback Mechanism in Depolarization-Induced Sustained Activation of Extracellular Signal-Regulated Kinase in the Hippocampus

Figure 3

KCl-induced sustained ERK activation requires receptor tyrosine kinase activity.

ERK activation in the hippocampal slices was examined 1 h (A, sustained) or immediately (B, immediate) after KCl treatment. Representative blots are shown in A1 and B1 and summary data are shown in A2 and B2 (n = 7 in both sets). ERK was activated in the control slices 1 h after KCl treatment. But, the receptor tyrosine kinase inhibitor (K252a) completely blocked ERK activation at this time point. The ERK activation immediately after the KCl stimulation was unaffected by K252a treatment (KCl and K252a + KCl, p>0.63). Compared to control, K252a did not affect the basal level of ERK phosphorylation (Sustained, p>0.08, Immediate, p>0.41). Asterisks denote significant difference (p<0.05).

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