Figure 1 | Scientific Reports

Figure 1

From: Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery

Figure 1

Nucleotide sequences for VAI-VAII region.

(a) VAI and VAII genes in the FVF fragment. VAI and VAII are boxed. prVAI-3 and prVAII-2 are the primers used for qPCR for the detection and quantification of VAI and VAII, respectively. Note that the actual R-primers are complementary to the sequences shown in this figure. pTP, terminal protein precursor; 52/55 k, 52 k and 55 k proteins. (b) B-box deletions of internal promoter in AxdV-4FVF-GFP and AxdV-4F-GFP. The deleted nucleotides are shown in small letters. (c) VAI-VAII deletion in AxdV-FVF-GFP and AxdV-F-GFP. The deleted region is shown in small letters. The deletion of 381 nt starts at 15 nt and 127 nt downstream from the 5′ end of VAI and VAII, respectively.

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