Figure 3 | Scientific Reports

Figure 3

From: Efficient production of adenovirus vector lacking genes of virus-associated RNAs that disturb cellular RNAi machinery

Figure 3

Viral genome and expressed VA-RNA of contaminated pre-vector replicating in 293 cells.

(a) Removal of the FVF fragment from the genome of the replicating pre-vector. Total DNAs of 293 cells infected with 15 μL of AxdV-4F-GFP stock were digested with EcoRV and the DNA fragment containing FVF and F was detected using a Southern blot technique. Other presentations are the same as in Fig. 1. (b) Detection of the expressed VA RNAs. Total RNAs of the same infected 293 cells were analyzed using a northern blot technique. 18 S, 18 S ribosomal RNA.

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