Figure 5
From: The α-helical regions of KERP1 are important in Entamoeba histolytica adherence to human cells
Analytical ultracentrifugation characterization of KERP1 and KCS.
Sedimentation velocity analysis of KERP1 (a and b) and KCS (c and d) at 20 µM. (a) Radial distribution of optical density measurements at 280 nm of KERP1: experimental data (dot) and fitted line (RMSD <0.006) to the continuous distribution model. (b) Sedimentation coefficient distribution c(s) showing a single peak compatible with a trimeric form of KERP1 with its hydrodynamic properties. (c) Radial distribution of optical density measurements at 280 nm of KCS experimental data (dot) and fitted line (RMSD <0.005) to the self-association monomer/trimer model. (d) Hydrodynamic properties of KCS monomer and trimer.
