Figure 1
From: Hierarchical molecular tagging to resolve long continuous sequences by massively parallel sequencing
Tile-seq basic procedure.
(a) Genomic DNA is targeted and amplified to include molecular barcodes. The barcodes are protected and subsequently exonuclease degraded. The reaction is sub-sampled and stopped at distinct intervals and indexed with a tag specific for the time of sampling. The molecules are circularized, fragmented and enriched for the junction between degraded and indexed ends. The junctions of degraded and indexed ends are amplified and sequenced. (b) Representation of the two PCRs incorporating a randomized clonal ID sequence and a known sample ID sequence. (c) Example agarose gel of eight samplings during exonuclease degradation of an amplicon showing the decreasing pool length of each TP. (d) The hierarchical structure given by three incorporated tags, sample id-tag (ID), clonal ID-tag (CID) and time point-tag (TP).
