Figure 5
From: An automated system for high-throughput single cell-based breeding
Single cell isolation of CHO cells.
(a) Cell array for CHO cells. Cells were stained with CytoRed solution. Scale bar = 160 μm. (b) Retrieval of single CHO cell. Scale bar = 25 μm. (c) Colony formation from isolated CHO cells. Scale bar = 200 μm. (d) On-chip culture of CHO cells. Cells were cultured on microchamber array chip and stained with PKH26. Scale bar = 100 μm. (e) Retrieval of cell cluster of CHO cells. CHO cells stained with PKH26 were trypsinized and retrieved with the robot. Scale bar = 25 μm. (f) Colony formation from isolated cell cluster of CHO cells. Scale bar = 200 μm.
