Figure 4
ClC-7 mediated transport driven by the activation of FaNaCh causes a remarkable increase of pH related fluorescence.
Effect of 30 µM FMRF on rpH on single cells expressing Ostm1-2AP-wtClC-7PM-E2GFP/DsRed and FaNaCh (a) or Ostm1-2AP-wtClC-7PM-E2GFP/DsRed alone (b). Scheme of the mechanism of the stimulation of ClC-7 transport by FaNaCh activation (c). Mean values of the changes of rpH at 100 seconds after the addition of FMRF (WT control: n = 31; WT FMRF: n = 31; WT+FaNaCh control: n = 24; WT+FaNaCh FMRF: n = 25; Error = SEM) (d).
