Table 1 Surveyor assay and sequencing for TALEN-induced mutations in Rat-1 fibroblasts
From: Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes
| Â | Â | Â | Sequence analysis | ||
|---|---|---|---|---|---|
Plasmid(s) | Cell No. (1 × 105) | Surveyor assay (%) | Mutations/Colonies | Mutation Rate (%) | Average del size (bp) |
GFP | 2.44 ± 0.58 | 0.44 ± 0.71 | — | — | — |
TALEN | 2.81 ± 0.23 | 6.06 ± 0.56 | 5/96 | 5.2 | 14.2 ± 6.5 |
TALEN-NC | 2.72 ± 0.33 | 11.04 ± 1.77 | 7/96 | 7.3 | 17.4 ± 6.8 |
TALEN-NC + Exo1 | 3.02 ± 0.35 | 25.86 ± 1.91 | 17/96 | 17.7 | 11.9 ± 2.6 |
