Figure 3
Expression levels of select Sirtuin substrates following treatment with Tnv-6.
Levels of p53, acetylated histone H4 (ac-H4) and acetylated tubulin (acet-tubulin) from 8 hour cultures of CLL cells with Tnv-6 were quantified using densitometry, standardized to respective β-actin values and expressed as the fold-change over untreated (control) cultures. In addition, phosphorylated H2AX (p-H2AX) levels were also quantified. Although heterogeneity of p53 and acet-H4 expression existed between patients, no significant difference in levels of any of the proteins was evident in Tnv-6-treated cells (Student's t-test on paired samples).
