Figure 6

Autophagy protein expression profiles following Tnv-6 treatment.

Compared to untreated (Control) cultures, Tnv-6-containing CLL cell cultures had increased lipidated LC3 (LC3-II, indicated by the lower band and arrow) (a). While a similar change in LC3-II was present in cells treated with bafilomycin-A (Baf-A), a synergistic increase in LC3-II was absent when Tnv-6 was combined with bafilomycin-A1 (b), in contrast to cultures with Ku-0063794 and bafilomycin-A1 (c). These data indicating a role for Tnv-6 as an autophagy-inhibitor are supported by the increase in cargo adaptor p62/Sequestosome following Tnv-6 treatment (d).