Figure 1
From: Tracking X-ray microscopy for alveolar dynamics in live intact mice
Schematic illustration of TrXM.
(a) Schematic of duct alveoli (DA) and sac alveoli (SA). (b) Overall lung movement during respiration. (c) Schematic view of tracking X-ray microscopy (TrXM). The alveolus at the apex top was tracked and maintained at one same coordinate in the field of view during respiration using motorized sample stage. The sample stage, a fast shutter and a lens-coupled charge-coupled device (CCD) camera were synchronized by a mechanical ventilator. SR: Synchrotron Radiation, M (left): Monochromator, FS: Fast Shutter, V: Ventilator, S: Scintillator, M (right): Mirror. (d) The acquisition sequences of alveolar visualization for microtomography. The pressure at the ventilator is shown at top. Projection images for microtomography were taken at the inspiratory (or expiratory) triggering during 180° rotation of the sample stage. The normal inspiration/expiration (I/E) ratio was taken as 1/2.
