Figure 1

ASBEL is transcribed from the DNA strand opposite to ANA/BTG3.

(a) Strand-specific RT-PCR analysis of ASBEL. (Upper panel) Schematic representation of the genomic organization of the region containing ASBEL and ANA/BTG3. Primers for reverse transcription (RT-primer) and PCR (PCR-primer) were designed to specifically target either the sense (Fw) or antisense strand (Rev1 and Rev2) of ASBEL. Oligo(dT)18 was used as a positive control. “Probe” indicates the region used for Northern blot analysis. (Lower panel) RT-PCR analysis was performed using the primers indicated. Sense RT-primers generated PCR products, whereas anti-sense RT-primers did not. (b) Northern blot analysis of ASBEL and GAPDH mRNA in JHOC5 cells. (c) Subcellular localization of ASBEL. JHOC5 cells were subjected to subcellular fractionation and the amounts of ASBEL in each fraction were evaluated by RT-PCR. GAPDH mRNA was used as a marker specific for the cytoplasm. U1 was used as a nuclear marker.