Figure 2

Exogenous TARS promotes angiogenesis in an in vitro endothelial tube formation assay and tube formation is sensitive to subnanomolar concentrations of BC194.

HUVECs were plated onto Matrigel^TM in Low serum (0.2% FBS) or EGM-2 Full serum media (2% FBS). Where indicated, 100 nM purified recombinant human TARS or 10 nM BC194 was added to the media. After 6 h, cells were fixed and stained with Oregon Green 488 Phalloidin. (a–d) are representative images; Scale bar = 100 μm. (e,f) show quantification of branches determined using the Simple Neurite Tracer plug-in on ImageJ software. Numbers represent average data from 3 separate experiments performed in duplicate. (e), Histogram of quantified branches for TARS effect; mean ± s.e.m., n = 3, *P < 0.01 compared to Low serum (LS) (Student's t-test). (f), Histogram of quantified branches for a range of BC194 concentrations added to Full serum media. Numbers represent mean ± s.e.m., n = 3, *P < 0.05 (one-way ANOVA, Tukey test).