Figure 5

TARS selectively induces migration, but not proliferation of endothelial cells.

(a) HUVECs were cultured in low serum (0.2% FBS) media containing 50 ng/ml VEGF and 10 nM BC194 where indicated; relative cell proliferation was measured over time using an alamarBlue® assay; mean ± s.e.m., n = 3, *P < 0.05 compared to Control (Student's t-test). (b,c) TARS induces endothelial cell migration. HUVEC migration was measured using a transwell assay. The migration compartment contained 50 ng/ml VEGF, 100 nM LARS or 100 nM TARS and 10 nM BC194 where indicated (b) Shown are representative images of DAPI stained nuclei from migrated cells after 4 h. (c) Histogram representing number of migrated cells after 4 h for the conditions indicated; mean ± s.e.m., n ≥ 3, *P < 0.05 compared to Control, #P < 0.05 compared to VEGF (one-way ANOVA, Tukey test).