Figure 2 | Scientific Reports

Figure 2

From: Characterization of Induced Neural Progenitors from Skin Fibroblasts by a Novel Combination of Defined Factors

Figure 2

Marker gene expression of neural progenitor cells in iNPCs and their differentiation potential.

(A) Transcriptional profiling of NPC-specific genes in iNPCs, Fbb as well as WT-NPCs was analyzed by SYBR-Green based quantitative RT-PCR with specific primer pairs (see Table 1). Error bars correspond to SEM. (B) The expression levels of fibroblast-specific genes in Fbb, iNPCs as well as WT-NPCs was analyzed by SYBR-Green based quantitative RT-PCR with specific primer pairs (see Table 1). GAPDH was used as an internal control. (C) iNPCs cultured on collagen IV-coated coverslips were incubated under neuronal, astrocyte and oligodendrocyte differentiation conditions, respectively and then subjected to immunostaining with polyclonal β-tubulin III (upper panel), monoclonal GFAP (middle panel) and polyclonal O4 (lower panel) antibodies and nuclear staining with DAPI (blue). (Scale bars: 50 μm).

Back to article page